Hello everyone, One of the issues I'm experiencing in my analysis is due to the low sequencing quality. I believe that the sequencing company to which I sent my samples does not have an optimized protocol for the reverse, as I'm seeing a very poor quality graph with the same pattern as another anal…

Hi @VerheulJulia , It seems like 260-217 is getting around 30% passed the filtering set and then almost all of the reads are merging from there. Although that is not ideal it is probably the best you can get with this quality. As for running single-end (just the R1 sequences) that could help get th…

QIIME 2 Forum

My sequences does not merge

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