I have some questions about qiime diversity adonis plugin (https://docs.qiime2.org/2019.4/plugins/available/diversity/adonis/). I have many metadata categories from which I would like to see how much % of variation they explain.
First question is, should I perform adonis test separately for categories a, b , c and d or should I inuclude all of them in the same calculation like this?
qiime diversity adonis
Secondly, I saw in another post that interaction between factors can be assessed using * instead of +. How can these interactions be proved? For example I did one test with + and another with * and the result was exactly the same (all results) except for one Pr(>F) (only from one factor). What does it mean? How are interactions detected?
Finally, I had a problem when running this plugin inside a pipeline. When running the command inside the pipeline I got one result and when I ran it alone got another different result. Any idea of what could be going on? Result should not be influenced for any other calculations, right? I didn’t found anything like this in the forum.
I hope everything is well explained,
Lots of thanks in advance!