I am trying to run longitudinal first-distances on a Jaccard distance matrix using the following command:
qiime longitudinal first-distances --i-distance-matrix DBA-core-metrics-results/jaccard_distance_matrix.qza --m-metadata-file metadatav.2.tsv --p-state-column Hour --p-individual-id-column studyID --p-replicate-handling random --o-first-distances DBA-first-distances-jacccard.qza
When I interactively explored the output artifact via
qiime metadata tabulate, I noticed that samples belonging to only five of the six timepoints were present (missing the first timepoint). I get the same result when using the
--p-baseline parameter, but for the sake of simplicity, I omitted it from the command above.
Attached is the initial metadata file used to execute the
longitudinal first-distances command as well as the metadata-tabulated
longitudinal first-distances output.
I suspect the problem lies in the initial metadata file and is user-mediated, but I have not been successful in my troubleshooting.
I appreciate your time and any suggestions!
P.S. I am currently running QIIME 2 2019.4, which was installed within a conda environment.