Missing one or more files for EMPSingleEndDirFmt: 'sequences.fastq.gz'

Dear all,
I am trying to import my single end reads into qiime, but with no success…everything seem right but I just don’t what’s happening. Kindly send help this way.

Here is the command and the error I keep getting:

qiime tools import
–type EMPSingleEndSequences
–input-path /home/elise/genohub_Namib_Elise/16S-ITS/ITS/New_folder
–output-path ./single-end-demux.qza

The error:

Missing one or more files for EMPSingleEndDirFmt: ‘sequences.fastq.gz’

I have also attached some screenshots. Kindly help.

Thanks,
Elise

h

image

Hi @elise_nghalipo,

It looks like your data is already demultiplexed. I’d recommend using the manifest format because I think it’s the easiest way to get your already demultiplexed data into QIIME 2.

Best,
Justine

1 Like

Hi @jwdebelius,

Thank you for your prompt response. When I use the sample manifest, this is what I get.

Thanks,
E

Hi @elise_nghalipo,

I linked you to documentation about the manifest format in my previous post, it looks like you may want to double check the import type in the docs and compare it to your import type.

Best,
Justine