# Missing Metadata

**URL:** https://forum.qiime2.org/t/missing-metadata/9332
**Category:** User Support
**Tags:** metadata
**Created:** [April 22, 2019, 12:15pm UTC](https://forum.qiime2.org/t/missing-metadata/9332 "2019-04-22T12:15:47Z")
**Posts on this page:** 1
**Showing post:** 6

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### Author: ![jwdebelius](https://forum.qiime2.org/user_avatar/forum.qiime2.org/jwdebelius/32/9655_2.png) [@jwdebelius](https://forum.qiime2.org/u/jwdebelius)
#### Post date: [April 24, 2019, 12:05pm UTC](https://forum.qiime2.org/t/missing-metadata/9332/6 "2019-04-24T12:05:53Z")

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It's necessary to have a set of samples that can answer your hypothesis. There are limitations to the statistical tests in QIIME that have discussed a fair bit on the forum. [Here's a recent discussion](https://forum.qiime2.org/t/comparing-beta-diversity-between-samples-not-groups/7981), although it can be helpful to search for others.

In general, I would expect a comparison of two samples per group to be underpowered to make statistically meaningful interpretation.

Best,  
Justine

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