Minimum Sample Count for Lefse Analysis

How many minimum samples do I need to perform lefse analysis?

I recently performed a lefse analysis with two sample sets, but no cladogram.

Please let me know

Thank you.

Hi @11126,
I am not too familiar with LEfSe and perhaps someone on this forum can lend their expertise, but in the meantime you may be able to recruit help much faster by posting directly on LEfSe’s developers forum as the QIIME 2 developers are not involved with LEfSe itself.

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Thank you! I’ll try it!