i really realy tired to trim my its2 data using itsx, and i got error : Error: QIIME 2 plugin 'itsxpress' has no action 'trim-pair-output-unmerged--i-per-sample-sequences'.

Welcome @Pena_Piana ! [image] Pena_Piana: ‘trim-pair-output-unmerged–i-per-sample-sequences’ Looks like you are just missing a space after "unmerged" Good luck!

Hi @Pena_Piana , Could you please report the full error message? See this line: [image] Pena_Piana: Debug info has been saved to /tmp/qiime2-q2cli-err-e9nwpqds.log Or re-run with the --verbose flag. Also, please do not post duplicate questions. You posted the same question twice, and I had …

thank you for your help. ... I got this error below, what I should do to fix this error message. thank you (qiime2-2020.6) afriana@alfriana-hp-laptop-14-cm0xxx:~/Documents/alfri$ qiime itsxpress trim-pair-output-unmerged --i-per-sample-sequences sequences.qza --p-region ITS2 --p-taxa F --o-trimmed…

Hi @Pena_Piana , That still does not look like the full error message, please note my requests above. In any case, I am not the itsxpress developer and cannot help troubleshoot, just trying to help you report the full necessary info. Maybe @Adam_Rivers could give you a hand if you report the full e…

@Pena_Piana My guess from this info would be that you need to update BBtools in your Qiime2 environment. With your Qiime environment active, run conda install -c bioconda bbmap. `

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