# Method to seperate FASTQ files containing 2 different amplicons

**URL:** https://forum.qiime2.org/t/method-to-seperate-fastq-files-containing-2-different-amplicons/12628
**Category:** Other Bioinformatics Tools
**Tags:** dada2
**Created:** [November 26, 2019, 12:56am UTC](https://forum.qiime2.org/t/method-to-seperate-fastq-files-containing-2-different-amplicons/12628 "2019-11-26T00:56:39Z")
**Posts on this page:** 2
**Page:** 1

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### Author: ![Bioaerosols](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/b/5f8ce5/32.png) [@Bioaerosols](https://forum.qiime2.org/u/Bioaerosols)
#### Post date: [November 26, 2019, 12:56am UTC](https://forum.qiime2.org/t/method-to-seperate-fastq-files-containing-2-different-amplicons/12628/1 "2019-11-26T00:56:39Z")

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Hi

I have a similar problem to this discussion - [Multi-gene amplicon sequences with dada2](https://forum.qiime2.org/t/multi-gene-amplicon-sequences-with-dada2/1402).  
Here Danny mentions there is code to split out each different gene in R and I'm wondering if anyone has this script or a similar one so I can run it with my FASTQ files to separate them, then use the suggestions posted above. I have 16S and ITS reads together in my FASTQ files.

Thanks

@Stream_biofilm

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [December 27, 2019, 6:56am UTC](https://forum.qiime2.org/t/method-to-seperate-fastq-files-containing-2-different-amplicons/12628/2 "2019-12-27T06:56:41Z")

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