Metadata file input

[Imee19]

Hi,
I’m new to QIIME2 and I have paired fastq.qz Miseq data. I am generating a metadata file for my run. I was just wondering what to input into the linker primer sequence column if I have forward and reverse primer sequences.

Thanks,
Imee19

[Nicholas_Bokulich]

Hi @Imee19,
That sequence column is not required, and I don’t even know of a QIIME 2 action that will typically use that column. The example metadata files we have in the tutorials have some columns like that just for data archiving purposes, not for functional reasons. So don’t worry about replicating that column in your own data! Alternatively, if you want to log this data in your metadata file, you can have both “ForwardPrimer” and “ReversePrimer” columns, and name them anything you like!

Good luck!