Hi,
When I run the qiime feature-table summarize command I get an error regarding my metadata file:
(qiime2-2018.6) lec49@bspc-laptop-013:~/Cleaner_Fish_Microbiome/16S_Run1/qiime_analysis$ qiime feature-table summarize --i-table table-dada2.qza --o-visualization table.qzv --m-sample-metadata-file metadata.csv
There was an issue with loading the file metadata.csv as metadata:
Found unrecognized ID column name 'sample-id,species,tissue-type,fish-size,gut-section' while searching for header. The first column name in the header defines the ID column, and must be one of these values:
Case-insensitive: 'feature id', 'feature-id', 'featureid', 'id', 'sample id', 'sample-id', 'sampleid'
Case-sensitive: '#OTU ID', '#OTUID', '#Sample ID', '#SampleID', 'sample_name'
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
Find details on QIIME 2 metadata requirements here: Metadata in QIIME 2 — QIIME 2 2018.6.0 documentation
I understand what the error is saying however my metadata file is formatted correctly, I have checked it using keemi with no problems reported and the header of the first column in 'sample-id'. The metadata file is attached.
Am I missing something?
metadata.csv (4.5 KB)