# Metabarcoding in diferents blocks

**URL:** <https://forum.qiime2.org/t/metabarcoding-in-diferents-blocks/33774>\
**Category:** Technical Support\
**Created:** [November 10, 2025, 7:24am UTC](https://forum.qiime2.org/t/metabarcoding-in-diferents-blocks/33774 "2025-11-10T07:24:43Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Cassiano\_Moreira](https://forum.qiime2.org/user_avatar/forum.qiime2.org/cassiano_moreira/32/19508_2.png) [@Cassiano\_Moreira](https://forum.qiime2.org/u/Cassiano_Moreira)\
**Post date:** [November 10, 2025, 7:24am UTC](https://forum.qiime2.org/t/metabarcoding-in-diferents-blocks/33774/1 "2025-11-10T07:24:44Z")

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I sequenced two blocks of samples in flowcell illumina on different dates, to perform in the ITS2 metabarcoding sequence in Qiime2. What is the best way to do the metabarcoding analyzes of these isolated blocks and then compile, since because they have sosos made on different dates they can be influenced by batches of reagents.

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**Author:** ![timanix](https://forum.qiime2.org/user_avatar/forum.qiime2.org/timanix/32/17879_2.png) [@timanix](https://forum.qiime2.org/u/timanix)\
**Post date:** [November 10, 2025, 7:28am UTC](https://forum.qiime2.org/t/metabarcoding-in-diferents-blocks/33774/2 "2025-11-10T07:28:37Z")

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Hello!

I believe [this topic](https://forum.qiime2.org/t/guidance-on-merging-and-handling-samples-from-multiple-sequencing-runs/32024) addresses your question.

Best,

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**Author:** ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)\
**Post date:** [December 11, 2025, 1:29pm UTC](https://forum.qiime2.org/t/metabarcoding-in-diferents-blocks/33774/3 "2025-12-11T13:29:16Z")

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