# Measure PERMANOVA on Bray-Curtis distance
**URL:** https://forum.qiime2.org/t/measure-permanova-on-bray-curtis-distance/27075
**Category:** User Support
**Created:** [July 14, 2023, 4:13pm UTC](https://forum.qiime2.org/t/measure-permanova-on-bray-curtis-distance/27075 "2023-07-14T16:13:26Z")
**Posts on this page:** 3
**Page:** 1
### Author:  [@Han\_Do](https://forum.qiime2.org/u/Han_Do)
#### Post date: [July 14, 2023, 4:13pm UTC](https://forum.qiime2.org/t/measure-permanova-on-bray-curtis-distance/27075/1 "2023-07-14T16:13:26Z")
Hi everyone,
I have error in run this code below. I use this similar code and metadata to measure PERMANOVA for unweighted and weighted UniFrac. I don't know what errors may come from for Bray-Curtis. Did I use incorrect codes?
Thank you so much for your help in my other posts!
qiime diversity beta-group-significance
--i-distance-matrix core-metrics-results/bray\_curtis\_distance\_matrix.qza
--m-metadata-file metadata\_VS.tsv
--m-metadata-column diseasextimexHTNo
--o-visualization core-metrics-results/bray-curtis-diseasextimexHTNo-significance.qzv
--p-pairwise
Usage: qiime diversity beta-group-significance [OPTIONS]
Determine whether groups of samples are significantly different from one
another using a permutation-based statistical test.
Inputs:
--i-distance-matrix ARTIFACT
DistanceMatrix Matrix of distances between pairs of samples.
[required]
Parameters:
--m-metadata-file METADATA
--m-metadata-column COLUMN MetadataColumn[Categorical]
Categorical sample metadata column. [required]
--p-method TEXT Choices('permanova', 'anosim', 'permdisp')
The group significance test to be applied.
[default: 'permanova']
--p-pairwise / --p-no-pairwise
Perform pairwise tests between all pairs of groups in
addition to the test across all groups. This can be
very slow if there are a lot of groups in the metadata
column. [default: False]
--p-permutations INTEGER
The number of permutations to be run when computing
p-values. [default: 999]
Outputs:
--o-visualization VISUALIZATION
[required]
Miscellaneous:
--output-dir PATH Output unspecified results to a directory
--verbose / --quiet Display verbose output to stdout and/or stderr during
execution of this action. Or silence output if
execution is successful (silence is golden).
--example-data PATH Write example data and exit.
--citations Show citations and exit.
--help Show this message and exit.
```
There were some problems with the command:
```
(1/3) Missing option '--m-metadata-file'.
(2/3) Missing option '--o-visualization'. ("--output-dir" may also be used)
(3/3) Got unexpected extra argument ( )
zsh: command not found: --m-metadata-column
---
### Author:  [@colinvwood](https://forum.qiime2.org/u/colinvwood)
#### Post date: [July 14, 2023, 8:18pm UTC](https://forum.qiime2.org/t/measure-permanova-on-bray-curtis-distance/27075/2 "2023-07-14T20:18:59Z")
Hello @Han_Do,
You probably didn't end the lines in the command:
> qiime diversity beta-group-significance
> --i-distance-matrix core-metrics-results/bray\_curtis\_distance\_matrix.qza
> --m-metadata-file metadata\_VS.tsv
> --m-metadata-column diseasextimexHTNo
> --o-visualization core-metrics-results/bray-curtis-diseasextimexHTNo-significance.qzv
> --p-pairwise
with backslashes, like so:
> qiime diversity beta-group-significance \
> --i-distance-matrix core-metrics-results/bray\_curtis\_distance\_matrix.qza \
> --m-metadata-file metadata\_VS.tsv \
> --m-metadata-column diseasextimexHTNo \
> --o-visualization core-metrics-results/bray-curtis-diseasextimexHTNo-significance.qzv \
> --p-pairwise
---
### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [August 15, 2023, 2:19am UTC](https://forum.qiime2.org/t/measure-permanova-on-bray-curtis-distance/27075/3 "2023-08-15T02:19:54Z")
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