Hello, All
I have created pe-33-manifest file for my ITS sequence data. I was getting this following error continuously
Could anyone please tell me how to rectify this error? I am also sharing with your my manifest file for your perusal.
Hi @Asha1,
The âPairedEndFastqManifestPhred33â format you are using requires 3 columns for each fastq:
sample-id,fastq.path,forward/reverse
The final indicating if the sequence file includes forward or reverse reads respectively.
Hope it helps,
Luca
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