Manifestfile error

Hello, All

I have created pe-33-manifest file for my ITS sequence data. I was getting this following error continuously

Could anyone please tell me how to rectify this error? I am also sharing with your my manifest file for your perusal.

Hi @Asha1,

The ‘PairedEndFastqManifestPhred33’ format you are using requires 3 columns for each fastq:

The final indicating if the sequence file includes forward or reverse reads respectively.
Hope it helps,

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