### Author:  [@Biancabrown](https://forum.qiime2.org/u/Biancabrown)
#### Post date: [September 14, 2017, 4:39pm UTC](https://forum.qiime2.org/t/manifest-import-issue/1238/1 "2017-09-14T16:39:05Z")
Hello,
I have an issue with importing my fastq files into the qiime format using the manifest option. .
Error Message
```bash
module: loading 'anaconda/3-4.4.0'
Traceback (most recent call last):
File "/users/bbrown3/anaconda/qiime2-2017.8/bin/qiime", line 6, in
sys.exit(q2cli. __main__.qiime())
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/click/core.py", line 722, in __call__
return self.main(*args, **kwargs)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/click/core.py", line 697, in main
rv = self.invoke(ctx)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/click/core.py", line 1066, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/click/core.py", line 1066, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/click/core.py", line 895, in invoke
return ctx.invoke(self.callback, **ctx.params)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/click/core.py", line 535, in invoke
return callback(*args, **kwargs)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/q2cli/tools.py", line 111, in import_data
view_type=source_format)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/qiime2/sdk/result.py", line 192, in import_data
return cls._from_view(type_, view, view_type, provenance_capture)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/qiime2/sdk/result.py", line 217, in _from_view
result = transformation(view)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/qiime2/core/transform.py", line 59, in transformation
new_view = transformer(view)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/q2_types/per_sample_sequences/_transformer.py", line 336, in _8
single_end=False)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/q2_types/per_sample_sequences/_transformer.py", line 266, in _fastq_manifest_helper
absolute=True)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/q2_types/per_sample_sequences/_transformer.py", line 156, in _parse_and_validate_manifest
_validate_paired_end_fastq_manifest_directions(manifest)
File "/users/bbrown3/anaconda/qiime2-2017.8/lib/python3.5/site-packages/q2_types/per_sample_sequences/_transformer.py", line 209, in _validate_paired_end_fastq_manifest_directions
'"reverse", but observed: %s' % direction)
ValueError: Directions can only be "forward" or "reverse", but observed: reverse`Preformatted text`
```
Qiime code:
```bash
qiime tools import --type 'SampleData[PairedEndSequencesWithQuality]' --input-path $PWD/scripts/pe-33-manifest.txt --source-format PairedEndFastqManifestPhred33 --output-path $PWD/demux-paired-end.qza
```
Below is the text file that that I use:
```plaintext
sample-id,absolute-filepath,direction
BB1,fastq/BB1_01_L001_R1_001.fastq,forward
BB1,fastq/BB1_01_L001_R2_001.fastq,reverse
BB10,/fastq/BB10_06_L001_R1_001.fastq,forward
BB10,/fastq/BB10_06_L001_R2_001.fastq,reverse
```
Any suggestions on how to fix this?
---
### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [September 14, 2017, 6:55pm UTC](https://forum.qiime2.org/t/manifest-import-issue/1238/3 "2017-09-14T18:55:14Z")
Hi @Biancabrown!
The error message you provided above says that in the last column it observed a value of
```auto
reverse`Preformatted text`
```
It looks to me like one of your entries in your manifest might not be `reverse` or `forward` in the `direction` column.
Would it be possible for you to copy-and-paste or attach your complete manifest file here? The sample you provided is invalid, too, but for different reasons (there are relative filepaths listed there, instead of absolute filepaths).
Thanks! 🦖
---
### Author:  [@Biancabrown](https://forum.qiime2.org/u/Biancabrown)
#### Post date: [September 14, 2017, 11:37pm UTC](https://forum.qiime2.org/t/manifest-import-issue/1238/5 "2017-09-14T23:37:21Z")
Hi @thermokarst this worked great. I had black a space at the end of one of the reverse.
Thanks!
---
### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [October 3, 2017, 3:47am UTC](https://forum.qiime2.org/t/manifest-import-issue/1238/6 "2017-10-03T03:47:30Z")
An off-topic reply has been merged into an existing topic: [How I will import Combined R1.fastq and R2.fastq files into QIIME2?](https://forum.qiime2.org/t/how-i-will-import-combined-r1-fastq-and-r2-fastq-files-into-qiime2/1382)
Please keep replies on-topic in the future.
---
### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [November 3, 2017, 9:47am UTC](https://forum.qiime2.org/t/manifest-import-issue/1238/9 "2017-11-03T09:47:44Z")
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