Manifest file importing

I am using qiime2/2021.11
Try to import the file
qiime tools import --type 'SampleData[SequencesWithQuality]' --input-path se-33-manifest --output-path single-end-demux.qza --input-format SingleEndFastqManifestPhred33V2

I downloaded the se-33-manifest file, and modified the first two lines for two files, it worked. However, when I copied the first two lines and pasted, and modified for two additional fastq files, it gave me this error:
"se-33-manifest is not a(n) SingleEndFastqManifestPhred33V2 file:

Missing filepath on line 3 and column "absolute-filepath"."

Does anyone know how to solve this problem? How to get an appropriate manifest file?

Thank you!


Based on the error you are facing, there is some issue on line 3 in the manifest file. Could you check if absolute filepath is indicated for third file? It is hard to troubleshoot without actual file, so If you'll fail to fix it, please share with us your manifest file.


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