I am using qiime2/2021.11
Try to import the file
qiime tools import --type 'SampleData[SequencesWithQuality]' --input-path se-33-manifest --output-path single-end-demux.qza --input-format SingleEndFastqManifestPhred33V2
I downloaded the se-33-manifest file, and modified the first two lines for two files, it worked. However, when I copied the first two lines and pasted, and modified for two additional fastq files, it gave me this error:
"se-33-manifest is not a(n) SingleEndFastqManifestPhred33V2 file:
Missing filepath on line 3 and column "absolute-filepath"."
Does anyone know how to solve this problem? How to get an appropriate manifest file?