Thank you for your response.
I have imported data in qiime2. It has demux.qza file was generated. File name is demux.qza
When I run second command to check quality of data. Error comes. Please, see command and error appered.
It is:
time qiime demux summarize
–i-data demux.qza
–o-visualization demux.qzv
Plugin error from demux:
[Errno 2] File b’/var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-archive-x9_doten/84a61338-9fc6-49e8-93e8-2204e040874b/data/MANIFEST’ does not exist: b’/var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-archive-x9_doten/84a61338-9fc6-49e8-93e8-2204e040874b/data/MANIFEST’
Debug info has been saved to /var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-q2cli-err-no_m0z3y.log
[Errno 2] File b'/var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-archive-gdlbtzat/f6af3b04-80ac-4bc2-9583-1748b05b6bf1/data/MANIFEST' does not exist: b'/var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-archive-gdlbtzat/f6af3b04-80ac-4bc2-9583-1748b05b6bf1/data/MANIFEST'
Debug info has been saved to /var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-q2cli-err-65amx5up.log
[Errno 2] File b'/var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-archive-78hd1qgf/f6af3b04-80ac-4bc2-9583-1748b05b6bf1/data/MANIFEST' does not exist: b'/var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-archive-78hd1qgf/f6af3b04-80ac-4bc2-9583-1748b05b6bf1/data/MANIFEST'
Debug info has been saved to /var/folders/1f/dgymh0l92xz6q91mqt0l2y800000gn/T/qiime2-q2cli-err-yl3yczgl.log
I am getting same error.
Is there any problem with MANIFEST file and metadata.yml file?
If yes, please share MANIFEST file and metadata.yml file formats.
Thanks for sharing @Bhagwan! It looks like you imported this by manually creating a SingleLanePerSamplePairedEndFastqDirFmt format, which is really impressive! Unfortunately, it looks like things went wrong, which isn’t surprising — this format is not really designed for people to create, but rather, for computers. Judging by your filenames, it would be easiest to use one of the Casava import formats.
Please give that a try and let us know how it goes.
Thank you @thermokars -Matthew Ryan Dillon.
Yep. I have good news. It’s working. I am learning qiime2. I will ask you for help if I stuck somewhere. Thank you once again.