looking for other conserved genes besides 16S

Hi everyone
I'm working with a large set of samples that we thought were bacterial isolates and instead it seems like we may have a mix of 2 or more Streptomyces species within 1 sample. I've been using ContEst16S | Ezbiocloud.net which looks for copies of the 16S genes. However, I want to be sure that I indeed have different Streptomyces species in the samples, and I've been recommended to look for other genes like S5 or orp. I've been recommended to use anv'io. Is there any other software out there that you recommend for this purpose?