This probably sounds silly, but here’s a thought:
imagine a scenario where you have two treatments showing different chemical results. However, both treatments suggest relative abundance of a specific phyla to be higher than others. In that case, it becomes difficult to discuss variation in the treatments from a biological perspective (without relying on chemical data). I was wondering if there was a way QIIME2 could integrate the amount of DNA present in the different samples (because it is obvious that bacteria grew more in the latter scenario); hence correcting the relative abundance by the amount of DNA (or cellular biomass) might give a more absolute quantification of community composition? … maybe?
If it’s too silly, feel free to delete the question! I actually have a similar scenario like that, so I was thinking how to solve this issue.