Hi,
I would like to ask for advise for my below problem:
(qiime2-2021.8) qiime2@qiime2core2021-8:~/MicrobiomeData$ qiime dada2 denoise-paired --i-demultiplexed-seqs raw_reads.qza --p-trim-left-f 0 --p-trim-left-r 0 --p-trunc-len-f 255 --p-trunc-len-r 200 --o-table table.qza --o-representative-sequences rep-seqs.qza --o-denoising-stats denoising-stats.qza --verbose
Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.
Command: run_dada_paired.R /tmp/tmp6cexws17/forward /tmp/tmp6cexws17/reverse /tmp/tmp6cexws17/output.tsv.biom /tmp/tmp6cexws17/track.tsv /tmp/tmp6cexws17/filt_f /tmp/tmp6cexws17/filt_r 255 200 0 0 2.0 2.0 2 12 independent consensus 1.0 1 1000000
R version 4.0.5 (2021-03-31)
Loading required package: Rcpp
DADA2: 1.18.0 / Rcpp: 1.0.7 / RcppParallel: 5.1.4
- Filtering ......................................................................................................
- Learning Error Rates
Error: cannot allocate vector of size 202.2 Mb
Execution halted
Warning message:
system call failed: Cannot allocate memory
Traceback (most recent call last):
File "/home/qiime2/miniconda/envs/qiime2-2021.8/lib/python3.8/site-packages/q2_dada2/_denoise.py", line 266, in denoise_paired
run_commands([cmd])
File "/home/qiime2/miniconda/envs/qiime2-2021.8/lib/python3.8/site-packages/q2_dada2/_denoise.py", line 36, in run_commands
subprocess.run(cmd, check=True)
File "/home/qiime2/miniconda/envs/qiime2-2021.8/lib/python3.8/subprocess.py", line 516, in run
raise CalledProcessError(retcode, process.args,
subprocess.CalledProcessError: Command '['run_dada_paired.R', '/tmp/tmp6cexws17/forward', '/tmp/tmp6cexws17/reverse', '/tmp/tmp6cexws17/output.tsv.biom', '/tmp/tmp6cexws17/track.tsv', '/tmp/tmp6cexws17/filt_f', '/tmp/tmp6cexws17/filt_r', '255', '200', '0', '0', '2.0', '2.0', '2', '12', 'independent', 'consensus', '1.0', '1', '1000000']' returned non-zero exit status 1.
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File "/home/qiime2/miniconda/envs/qiime2-2021.8/lib/python3.8/site-packages/q2cli/commands.py", line 329, in call
results = action(**arguments)
File "", line 2, in denoise_paired
File "/home/qiime2/miniconda/envs/qiime2-2021.8/lib/python3.8/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
outputs = self.callable_executor(scope, callable_args,
File "/home/qiime2/miniconda/envs/qiime2-2021.8/lib/python3.8/site-packages/qiime2/sdk/action.py", line 391, in callable_executor
output_views = self._callable(**view_args)
File "/home/qiime2/miniconda/envs/qiime2-2021.8/lib/python3.8/site-packages/q2_dada2/_denoise.py", line 279, in denoise_paired
raise Exception("An error was encountered while running DADA2"
Exception: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
See above for debug info.
It seems to me that the VirtualBox has limited memory. I need to run large data file (4-6 Gb per sequencing run), so I am not sure if I can proceed to use the QIIME2 on VirtualBox. I am very sorry, but I am new to this. Thank you very much for any advise!