# large ITS dataset

**URL:** https://forum.qiime2.org/t/large-its-dataset/28695
**Category:** PICRUSt2
**Tags:** its, picrust
**Created:** [December 21, 2023, 3:00pm UTC](https://forum.qiime2.org/t/large-its-dataset/28695 "2023-12-21T15:00:05Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![Sue](https://forum.qiime2.org/user_avatar/forum.qiime2.org/sue/32/16450_2.png) [@Sue](https://forum.qiime2.org/u/Sue)
#### Post date: [December 21, 2023, 3:00pm UTC](https://forum.qiime2.org/t/large-its-dataset/28695/1 "2023-12-21T15:00:05Z")

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Hi,  
I have tested Qiime2 on a mock ITS dataset ([DNA metabarcoding of a mock community of 189 fungal strains associated to plants and soils. - Omics](https://entrepot.recherche.data.gouv.fr/dataset.xhtml;jsessionid=34ec77dbde5e1eff44a80c6f1c20?persistentId=doi%3A10.15454%2F8CVWRR&version=&q=&fileAccess=Public&fileTag=%22Data%22&fileSortField=&fileSortOrder=)). Subsequently, I applied Picrust2, and now I want to visualize the results using ggPicrust2. However, my mock data is not large enough to pass the statistical method LinDa. Therefore, I hope you can share some ITS fastq files and the associated metadata file with me. I also found this repository, but I am unsure how to download the fastq files: [GitHub - caporaso-lab/mockrobiota: A public resource for microbiome bioinformatics benchmarking using artificially constructed (i.e., mock) communities.](https://github.com/caporaso-lab/mockrobiota)

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### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [December 27, 2023, 7:45pm UTC](https://forum.qiime2.org/t/large-its-dataset/28695/2 "2023-12-27T19:45:40Z")

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Hello Fletcher,

On GitHub, it's probably easiest to download the full repo to get the fastq files you need:

Click Code \> Download ZIP

 ![Screenshot 2023-12-27 at 11.42.03 AM](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/d/3/d3eb43681cc55c26e5141698667e9ef4fdc504fd.png)

Warning: PICTURSt2 is not magic and is limited what's in the database. But it's still worth a try!

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [January 28, 2024, 1:46am UTC](https://forum.qiime2.org/t/large-its-dataset/28695/3 "2024-01-28T01:46:34Z")

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