I have imported ITS samples into qiime2 using this command:
qiime tools import
–type SampleData[PairedEndSequencesWithQuality]
–input-format PairedEndFastqManifestPhred33
–input-path manifest.txt
–output-path sequences.qza
Now which one of the below should I use to extract the ITS1 regions, what is use of p-cluster-id 1.0?
Hi @Yogesh_Gupta,
You can see the q2-ITSxpress github page for usage details and descriptions of these parameters, or use the help documentation (qiime itsxpress trim-pair-output-unmerged --help) to get the same information.