@ewmorr I have added a --reversed-primers option to itsxpress and q2-itsxpress. I still need to write some tests before I version it and release it. If you want to try it out prerelease you can. Create a new Qiime conda environment according to their instructions then run conda install hmmer bbmap vsearch. Install itsxpress and q2-itsxpress with git (git clone https://github.com/USDA-ARS-GBRU/q2_itsxpress.git and git clone https://github.com/USDA-ARS-GBRU/itsxpress.git) and switch to the branch issue_11 for itsxpress and reversed_primers for q2-itsxpress. Finally cd into the q2-itsxpress and itsxpress directories and run pip install -e . in each one.
3 Likes