# Issues with Mr DNA's FastQ Processor
**URL:** https://forum.qiime2.org/t/issues-with-mr-dnas-fastq-processor/19311
**Category:** Other Bioinformatics Tools
**Created:** [April 21, 2021, 4:49am UTC](https://forum.qiime2.org/t/issues-with-mr-dnas-fastq-processor/19311 "2021-04-21T04:49:58Z")
**Posts on this page:** 3
**Page:** 1
### Author:  [@bkramer](https://forum.qiime2.org/u/bkramer)
#### Post date: [April 21, 2021, 4:49am UTC](https://forum.qiime2.org/t/issues-with-mr-dnas-fastq-processor/19311/1 "2021-04-21T04:49:58Z")
Hello,
I am having trouble using the app Mr DNA has to remove forward and reverse primer sequences from my raw, demultiplexed data. I follow the instructions (presumably quite well) that Mr DNA provides, yet for some reason when I attempt to combine all of my R1 and R2 files together and remove the primer sequences, it only generates a single empty "demux" folder and a sample-metadata.tsv files. Has anyone encountered this problem before?
Ben
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### Author:  [@ijgretch](https://forum.qiime2.org/u/ijgretch)
#### Post date: [March 6, 2023, 4:24pm UTC](https://forum.qiime2.org/t/issues-with-mr-dnas-fastq-processor/19311/2 "2023-03-06T16:24:40Z")
I (a first-time doer of bioinformatics) am currently having exactly the same issue. Did you ever figure this out and/or find another better way to remove the primer sequences?
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### Author:  [@ijgretch](https://forum.qiime2.org/u/ijgretch)
#### Post date: [March 8, 2023, 6:12pm UTC](https://forum.qiime2.org/t/issues-with-mr-dnas-fastq-processor/19311/3 "2023-03-08T18:12:17Z")
Found the problem. I am not afraid to admit that I forgot to unzip the files. I was trying to do this with fastq.gz files, not fastq files, whoops. Worked like a charm after that.