I am new to both bioinformatics and Qiime entirely, i was able to run the moving picture turtorial successfully. now working arounf my data, running the following command
qiime diversity beta-group-significance --i-distance-matrix core-metrics-results/unweighted_unifrac_distance_matrix.qza --m-metadata-file inoculum2-sample-metadata.tsv --m-metadata-column sampleid --o-visualization core-metrics-results/unweighted-unifrac-body-site-significance.qzv --p-pairwise
gives this error ( (1/1) Invalid value for '--m-metadata-file': There was an issue with
retrieving column 'sampleid' from the metadata.).
i read up other issues in the forum but couldnt still get it, although i changed to a different colunm (Sample-number-or-well-position) after reading several issues then came up with a different error (Plugin error from diversity:
All values in the grouping vector are unique. This method cannot operate on a grouping vector with only unique values (e.g., there are no 'within' distances because each group of objects contains only a single object).
Debug info has been saved to /tmp/qiime2-q2cli-err-gqt1ixaz.log). Attached is my metadata sample-metadata.tsv (4.1 KB)