# Issue importing Paired end sequences with quality

**URL:** https://forum.qiime2.org/t/issue-importing-paired-end-sequences-with-quality/21256
**Category:** Technical Support
**Tags:** import, fastq-manifest
**Created:** [November 4, 2021, 5:49pm UTC](https://forum.qiime2.org/t/issue-importing-paired-end-sequences-with-quality/21256 "2021-11-04T17:49:03Z")
**Posts on this page:** 1
**Showing post:** 2

<div class="post-metadata">

### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [November 5, 2021, 2:20pm UTC](https://forum.qiime2.org/t/issue-importing-paired-end-sequences-with-quality/21256/2 "2021-11-05T14:20:17Z")

</div>

Hello Lautaro,

I took a look at your manifest file, and it looks good, except for the line endings.

(Windows and Linux use different ways to note a  
new  
line  
of  
text.)

Once you convert to the Linux format by saving it with a text editor or using a command like  
`dos2unix Manifest.txt`  
then this should work great!

You are not the first to have this issue! More info here:

> [@FASTQ Files Importing Error](https://forum.qiime2.org/t/fastq-files-importing-error/6412/19):
>
> If you use Excel to make your CSV, it will insert CRLF line breaks (whether or not you are using windows, I believe). So you need to either just open in textedit and use find/replace to fix the line breaks, or use mac2unix or a program like that to convert the carriage returns. So Excel is okay — just make sure to convert the line endings. This will be an issue with making sample metadata files, too, not only manifest files.

---

_[View the full topic](https://forum.qiime2.org/t/issue-importing-paired-end-sequences-with-quality/21256)._
