issue importing manifest.tsv in Parkinson’s Mouse Tutorial

Hi! I'm going through the Parkinson’s Mouse Tutorial and having an issue when importing the manifest.tsv file. I have q2cli version 2022.11.1 and installed it using conda.

these are the exact commands i run:
-O "manifest.tsv"

-O ""


qiime tools import
--type "SampleData[SequencesWithQuality]"
--input-format SingleEndFastqManifestPhred33V2
--input-path ./manifest.tsv
--output-path ./demux_seqs.qza

my error is:
" There was a problem importing ./manifest.tsv:

manifest.tsv is not a(n) SingleEndFastqManifestPhred33V2 file:

Filepath on line 1 and column "absolute-filepath" could not be found ($PWD/demultiplexed_seqs/10483.recip.220.WT.OB1.D7_30_L001_R1_001.fastq.gz) for sample "recip.220.WT.OB1.D7"."

this is what my directory looks like:


Can you try again, being sure you are in the directory where you downloaded the manifest and the demultiplexed sequences?

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