Hi! I'm going through the Parkinson’s Mouse Tutorial and having an issue when importing the manifest.tsv file. I have q2cli version 2022.11.1 and installed it using conda.
these are the exact commands i run:
wget
-O "manifest.tsv"
"https://data.qiime2.org/2022.11/tutorials/pd-mice/manifest"
wget
-O "demultiplexed_seqs.zip"
"https://data.qiime2.org/2022.11/tutorials/pd-mice/demultiplexed_seqs.zip"
unzip demultiplexed_seqs.zip
qiime tools import
--type "SampleData[SequencesWithQuality]"
--input-format SingleEndFastqManifestPhred33V2
--input-path ./manifest.tsv
--output-path ./demux_seqs.qza
my error is:
" There was a problem importing ./manifest.tsv:
manifest.tsv is not a(n) SingleEndFastqManifestPhred33V2 file:
Filepath on line 1 and column "absolute-filepath" could not be found ($PWD/demultiplexed_seqs/10483.recip.220.WT.OB1.D7_30_L001_R1_001.fastq.gz) for sample "recip.220.WT.OB1.D7"."
this is what my directory looks like: