I am currently using the Differential Abundance Analysis with gneiss, and am having issues generating the regression_summary.qzv.
I was able to generate the hierarchy.qza and balances.qza, but have issues when I get to the regression step.
It seems to be an issue with my metadata file. As I keep getting this error:
Plugin warning from gneiss:
ols-regression is deprecated and will be removed in a future version of this pl$
warnings.warn(‘Cannot determine yajl version, assuming <1.0.12’)
Plugin error from gneiss:
Error evaluating factor: NameError: name ‘sampleID’ is not defined
I have been searching on all forum posts and it seems others had this issue because of blank spaces in their metadata file, not having sampleid column, and the metadata file not being a tsv file. I have addressed these issues and I am not sure what is wrong at this point. Any insights?
I am using qiime2 version 2019.10 installed with conda.
This is the code that I ran:
qiime gneiss ols-regression
I verified my metadata file with Keemei and it checked out. Let me know if you would like me to attach that.
Thank you for your time!