# Is unrestricted taxonomic assignment better for estimating numbers of genera? **URL:** https://forum.qiime2.org/t/is-unrestricted-taxonomic-assignment-better-for-estimating-numbers-of-genera/27762 **Category:** General Discussion **Tags:** taxonomy, feature-classifier **Created:** [September 25, 2023, 2:18pm UTC](https://forum.qiime2.org/t/is-unrestricted-taxonomic-assignment-better-for-estimating-numbers-of-genera/27762 "2023-09-25T14:18:50Z") **Posts on this page:** 1 **Showing post:** 11
### Author: ![Jan\_Kollar](https://forum.qiime2.org/user_avatar/forum.qiime2.org/jan_kollar/32/19240_2.png) [@Jan\_Kollar](https://forum.qiime2.org/u/Jan_Kollar) #### Post date: [September 27, 2023, 4:52pm UTC](https://forum.qiime2.org/t/is-unrestricted-taxonomic-assignment-better-for-estimating-numbers-of-genera/27762/11 "2023-09-27T16:52:32Z")
Hi @SoilRotifer, > [@SoilRotifer](#): > > This made me realize that I should have suggested that you try using the [weighted classifiers](https://forum.qiime2.org/t/pretrained-silva-138-1-classifiers-weighted-and-uniform-reference-dbs-and-habitat-weights/22627), these are also available on the [Data resources page](https://docs.qiime2.org/2023.7/data-resources/#weighted-taxonomic-classifiers)... Very interesting suggestion and an excellent explanation, will definitely try that, thank you! > [@SoilRotifer](#): > > Perhaps you can align these amplicons to some reference genera and see how they phylogenetically cluster? Did that, helped with resolving some ASVs at genus level (and, interestingly, the unrestricted classification was correct there at the genus level as well! Plus BLAST also suggested the same as the top hit, so yay!). > [@SoilRotifer](#): > > You can also use [RESCRIPt](https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494/2) to curate your own reference database. For example, you might consider some of the other options for the `qiime rescript dereplicate ...` command compared to what is outlined in the tutorial. Actually, I used your excellent [tutorial](https://forum.qiime2.org/t/using-rescripts-extract-seq-segments-to-extract-reference-sequences-without-pcr-primer-pairs/23618) while building my custom 18S diatom reference database, along with [this one](https://forum.qiime2.org/t/using-rescript-to-compile-sequence-databases-and-taxonomy-classifiers-from-ncbi-genbank/15947). Extremely useful, thank you! I'll check also the one you suggest. > [@SoilRotifer](#): > > This sounds like a really cool project! Reminds me of my micro-invertebrate days. 🙂 Yeah, it's very small, very simple (relatively), and a lot of fun. With 113 ASVs, it's possible to basically pay attention to every one of them, which is kind of cool, especially compared to working with bacteria. Soon, I'll have to move to our global dataset with several hundreds of samples and I have some feeling that I'll look back to these humble beginnings with tears of pain in my eyes 😃 Anyway, thank you very much for your time and ideas, it's a great help! --- _[View the full topic](https://forum.qiime2.org/t/is-unrestricted-taxonomic-assignment-better-for-estimating-numbers-of-genera/27762)._