# Is there a way to use a FASTA/QUAL file for the moving pictures tutorial?

**URL:** https://forum.qiime2.org/t/is-there-a-way-to-use-a-fasta-qual-file-for-the-moving-pictures-tutorial/5093
**Category:** User Support
**Created:** [July 17, 2018, 6:50pm UTC](https://forum.qiime2.org/t/is-there-a-way-to-use-a-fasta-qual-file-for-the-moving-pictures-tutorial/5093 "2018-07-17T18:50:52Z")
**Posts on this page:** 1
**Showing post:** 2

<div class="post-metadata">

### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [July 17, 2018, 7:06pm UTC](https://forum.qiime2.org/t/is-there-a-way-to-use-a-fasta-qual-file-for-the-moving-pictures-tutorial/5093/2 "2018-07-17T19:06:47Z")

</div>

> [@spark](#):
>
> I have used QIIME 1 to convert my FASTA/QUAL file into a FASTQ file

👍

There is no way to use fasta+qual files, but you did the right first step (this is an old format so we don't really intend to support direct importation of this format)

> [@spark](#):
>
> I only have a FASTQ file for the sequences and am lacking one for the barcodes, so I am unable to proceed with the single file in the Moving Pictures tutorial.

where are the barcodes?

1. inline in the read  
Use [q2-cutadapt](https://forum.qiime2.org/t/demultiplexing-and-trimming-adapters-from-reads-with-q2-cutadapt/2313) to demultiplex
2. in the fastq header lines  
use other commands in qiime1 to create a separate barcode (maybe extract\_barcodes.py)
3. in a separate fasta for which there are no qual scores  
make fake qual scores for each barcode sequence.
4. you have no barcodes because this is one sample  
[import](https://docs.qiime2.org/2018.6/tutorials/importing/#fastq-manifest-formats) as `SampleData[SequencesWithQuality]` or `SampleData[PairedEndSequencesWithQuality]` format, which are for demultiplexed data, and proceed with denoising/otu clustering.

If the barcodes are in a different place, let us know.

---

_[View the full topic](https://forum.qiime2.org/t/is-there-a-way-to-use-a-fasta-qual-file-for-the-moving-pictures-tutorial/5093)._