Is there a way to use a FASTA/QUAL file for the moving pictures tutorial?

[Nicholas_Bokulich]

@spark

I have used QIIME 1 to convert my FASTA/QUAL file into a FASTQ file

There is no way to use fasta+qual files, but you did the right first step (this is an old format so we don't really intend to support direct importation of this format)

@spark

I only have a FASTQ file for the sequences and am lacking one for the barcodes, so I am unable to proceed with the single file in the Moving Pictures tutorial.

where are the barcodes?

1. inline in the read
   Use q2-cutadapt to demultiplex
2. in the fastq header lines
   use other commands in qiime1 to create a separate barcode (maybe extract_barcodes.py)
3. in a separate fasta for which there are no qual scores
   make fake qual scores for each barcode sequence.
4. you have no barcodes because this is one sample
   import as SampleData[SequencesWithQuality] or SampleData[PairedEndSequencesWithQuality] format, which are for demultiplexed data, and proceed with denoising/otu clustering.

If the barcodes are in a different place, let us know.