Hello, After allocating more RAM to my virtualbox on my PC, I was able to successfully complete the Data2 denoise command, albeit I let it run for 6 days. I now have table.qza and a rep-seqs.qza file. I am following the Moving Pictuers tutorial with my own data and now I need to perform the feature…

Hi @Lisa_Crummett ! [image] Lisa_Crummett: I was able to successfully complete the Data2 denoise Yay! [image] Lisa_Crummett: . I do not have a metadata and I thought that I didn’t need to create one as I imported a manifest file and sample data file in my first step The manifest forma…

Thank you so much, this does clarify things for me. I did have a look at the Keemei instructions and it says that the second column in your metadata file should be the barcode sequence and the third column should be the linker-primer sequence. I am confused because each of my samples is associated w…

By the way, my data is already demultiplexed so maybe I don't need the "barcode sequence" and "linker-primer sequence" columns?

Hi @Lisa_Crummett , sorry about the Linker Primer Sequence and Barcode confusion --- keemei was originally written for QIIME 1, which had those requirements. You can use keemei to validate QIIME 2 metadata files by selecting the following option: [05 PM] QIIME 2 metadata files don't have the same …

Thanks. The scree capture that you attached is not familiar to me. When I am running Qiime2 in virtualbox, I dont' have a tool bar like that with "Tools, Add-ons" where I can select "Keemei" and then "validate with Qiime2". I may just try running the command with my new metadata file. Can the metada…

Yay, it worked! I am able to look at my visualization files after running the commands for summarizing and tabulating my metadata. It's really cool!

[image] Lisa_Crummett: The scree capture that you attached is not familiar to me. Please see the link to keemei I provided above --- it is a Google Sheets add-on! [image] Lisa_Crummett: Can the metadata file be .csv or does it have to be a .tsv file? Metadata must be TSV (for now). …

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