# Is it possible to check a specific gene level in a specific genus?
**URL:** https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963
**Category:** User Support
**Tags:** metagenomics
**Created:** [January 19, 2024, 8:17am UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963 "2024-01-19T08:17:42Z")
**Posts on this page:** 11
**Page:** 1
### Author:  [@sooni](https://forum.qiime2.org/u/sooni)
#### Post date: [January 19, 2024, 8:17am UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/1 "2024-01-19T08:17:42Z")
Hello. I always thanks for your help.
I want to check a specific gene level in a specific genus.
Is it possible with QIIME2-Shotgun distribution?
If possible, how can I do it?
I'm sorry if this may be a topic that overlaps with the posts I've posted so far.
Thank you for your help!
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### Author:  [@colinvwood](https://forum.qiime2.org/u/colinvwood)
#### Post date: [January 19, 2024, 10:49pm UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/2 "2024-01-19T22:49:10Z")
Hello @sooni,
Could you explain what you mean by gene level?
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### Author:  [@sooni](https://forum.qiime2.org/u/sooni)
#### Post date: [January 19, 2024, 11:01pm UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/3 "2024-01-19T23:01:46Z")
A specific gene level means gene expression level of a specific gene.
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### Author:  [@colinvwood](https://forum.qiime2.org/u/colinvwood)
#### Post date: [January 19, 2024, 11:02pm UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/4 "2024-01-19T23:02:42Z")
Hello @sooni,
Are you working with RNA sequences?
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### Author:  [@sooni](https://forum.qiime2.org/u/sooni)
#### Post date: [January 20, 2024, 12:59am UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/5 "2024-01-20T00:59:39Z")
Yes. I am working with RNA sequences. Exactly, I am working with shotgun sequencing data.
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### Author:  [@gregcaporaso](https://forum.qiime2.org/u/gregcaporaso)
#### Post date: [January 22, 2024, 5:22pm UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/6 "2024-01-22T17:22:05Z")
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### Author:  [@colinvwood](https://forum.qiime2.org/u/colinvwood)
#### Post date: [January 22, 2024, 6:21pm UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/7 "2024-01-22T18:21:22Z")
Hello @sooni,
I believe you can still import your RNA sequencing data as dna sequences in the typical fashion (see the [importing docs](https://docs.qiime2.org/2023.9/tutorials/importing/) for more information).
Then, if you want to do targeted analysis you could use dada2 or `qiime vsearch dereplicate`, then `qiime vsearch cluster-features-closed-reference` with your genes of interest.
Or, if you want to do gene discovery you could use `qiime assembly assemble-meaghit` -\> `qiime moshpit eggnog*` in the shotgun distribution.
Please note that I am not very familiar with RNA sequencing data and the above is just a rough suggestion. Please also note that the functionality in the shotgun distribution is still in an alpha state and not fully documented yet.
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### Author:  [@sooni](https://forum.qiime2.org/u/sooni)
#### Post date: [January 25, 2024, 6:24am UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/12 "2024-01-25T06:24:05Z")
Thanks for your reply.
If I come across something I don't know during the analysis, can I ask more?
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### Author:  [@cherman2](https://forum.qiime2.org/u/cherman2)
#### Post date: [January 25, 2024, 5:43pm UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/13 "2024-01-25T17:43:29Z")
Hi @sooni,
You are always welcome to ask more questions as your work through the analysis. If its a follow-up question feel free to post on this thread, but if its a different isssue make sure to make a new topic!
Hope to see you around the :qiime2: forum soon!
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### Author:  [@cherman2](https://forum.qiime2.org/u/cherman2)
#### Post date: [January 25, 2024, 5:43pm UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/14 "2024-01-25T17:43:33Z")
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [February 25, 2024, 11:43pm UTC](https://forum.qiime2.org/t/is-it-possible-to-check-a-specific-gene-level-in-a-specific-genus/28963/15 "2024-02-25T23:43:43Z")
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