Invalid character 'a' (does not match IUPAC characters for this sequence type). Allowed characters are ACGTRYKMSWBDHVN.

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Hi @Bertram_Ji ,

You can use the approach outlined here for importing and converting mixed case sequences into upper-case. That is, your command would look like:

qiime tools import \
    --input-path PIDwholefract_otus97num.fasta \
    --type 'FeatureData[Sequence]' \
    --input-format 'MixedCaseDNAFASTAFormat' \
    --output-path PIDwholefract_otus97num.qza

Give that a try and let us know how it goes.

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