Thank you. However, I have another problem with the sample metadata. I received my sequences demultiplexed, is there any way I can create a metadata file without barcodes and linker sequences? The purpose of my research is to investigate the microbial community structure of Holocene and Pleistocene age permafrost samples (before and after the ice age). In my metadat file I included the name of the samples, age and depth. Do you think these information are enough for QIIME 2 to start the diversity analyses? I would appreciate if you help me with this question.
Thank you