Importing trimmed paired .fa

User Support
1

Hi All,

Is there a import path for paired trimmed data off of the CLC Workbench in .fa format?

Thanks

L

M00505:104:000000000-BG98H:1:1101:15264:1140_1:N:0:9/M00505:104:000000000-BG98H:1:1101:15264:1140_2:N:0:9
ACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCAGTCTGTTCAGGGTTCCAAACTCAA
CGATGGCAACTAAACACGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACACCTTACGGC
ACGAGCTGACGACAGCCATGCACCACCTGTGTCCGCGTTCCCGAAGGCACCCCTCTCTTT
CAAGAGGATTCGCGGCATGTCAAGCCCTGGTAAGGTTCTTCGCTTTGCATCGAATTAAAC
CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAAC
GTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACAGCACTGCACGGGTCGATACGCAC
AGCGCCTAGTATCCATCGTTTACGGCTAGGACTACTGGGGTATCTAATCCTGTT
M00505:104:000000000-BG98H:1:1101:16188:1172_1:N:0:9/M00505:104:000000000-BG98H:1:1101:16188:1172_2:N:0:9
ACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCAGTCTGTTCAGGGTTCCAAACTCAA
CGATGGCAACTAAACACGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACACCTTACGGC
ACGAGCTGACGACAGCCATGCACCACCTGTGTCCGCGTTCCCGAAGGCACCCCTCTCTTT
CAAGAGGATTCGCGGCATGTCAAGCCCTGGTAAGGTTCTTCGCTTTGCATCGAATTAAAC
CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAAC
GTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACAGCACTGCACGGGTCGATACGCAC
AGCGCCTAGTATCCATCGTTTACGGCTAGGACTACTGGGGTATCTAATCTTGTT

3

I think probably not — I am assuming that these are not yet demultiplexed (in which case no).

You should import the raw fastq data instead

However, if these reads are already demultiplexed and these are just fasta sequences associated with features in a feature table (in this case observation matrix of observed sequence counts per sample), import as described here

Good luck!

5

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