# Importing paired end demultiplexed MiSeq data **URL:** \ **Category:** User Support\ **Tags:** pending-development, paired-end, import, fastq-manifest\ **Created:** [February 13, 2018, 4:50pm UTC](https://forum.qiime2.org/t/importing-paired-end-demultiplexed-miseq-data/3014 "2018-02-13T16:50:05Z")\ **Posts on this page:** 1\ **Showing post:** 3
**Author:** ![David\_Bradshaw](https://forum.qiime2.org/user_avatar/forum.qiime2.org/david_bradshaw/32/6705_2.png) [@David\_Bradshaw](https://forum.qiime2.org/u/David_Bradshaw)\ **Post date:** [February 14, 2018, 5:21pm UTC](https://forum.qiime2.org/t/importing-paired-end-demultiplexed-miseq-data/3014/3 "2018-02-14T17:21:20Z")
Dear Mehrbod Estaki, Thank you very much. They are samples of the same areas a couple times over a period of time, I just made the manifest file with appropriate sample labels delineating sampling periods and it seems to be working fine. My next step is that I was going to follow [Analyzing paired end reads in QIIME 2](https://forum.qiime2.org/t/analyzing-paired-end-reads-in-qiime-2/2021) through the quality filtering step and then move on to de novo chimera checking and then OTU picking using the latest SILVA database (128). I also have to train the classifier correct? Would just use the following plugins? [uchime-denovo: De novo chimera filtering with vsearch. — QIIME 2 2017.12.0 documentation](https://docs.qiime2.org/2017.12/plugins/available/vsearch/uchime-denovo/) [Training feature classifiers with q2-feature-classifier — QIIME 2 2017.12.0 documentation](https://docs.qiime2.org/2017.12/tutorials/feature-classifier/) [cluster-features-de-novo: De novo clustering of features. — QIIME 2 2017.12.0 documentation](https://docs.qiime2.org/2017.12/plugins/available/vsearch/cluster-features-de-novo/) Thank you for your time and help, Sincerely, David Bradshaw --- _[View the full topic](https://forum.qiime2.org/t/importing-paired-end-demultiplexed-miseq-data/3014)._