# Importing .fna and workflow

**URL:** https://forum.qiime2.org/t/importing-fna-and-workflow/21109
**Category:** User Support
**Created:** [October 19, 2021, 5:02am UTC](https://forum.qiime2.org/t/importing-fna-and-workflow/21109 "2021-10-19T05:02:04Z")
**Posts on this page:** 5
**Page:** 1

<div class="post-metadata">

### Author: ![Fra](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/f/a6a055/32.png) [@Fra](https://forum.qiime2.org/u/Fra)
#### Post date: [October 19, 2021, 5:02am UTC](https://forum.qiime2.org/t/importing-fna-and-workflow/21109/1 "2021-10-19T05:02:04Z")

</div>

Hello everyone,

I have a single .fna file containing 118189 sequences in the following format (examples at the end of the message).

While I managed to import this file using the command:

```auto
qiime tools import \
--input-path rep_set.fna \
--output-path qiime2/seq.qza \
--type 'FeatureData[Sequence]'

```

I am unable to move forward in the analyses. Also, while this file looks like an output of DADA2, the sequences are not merged and have primers.

Do you guys have any recommendations?

Example of a sequence within the .fna file:

> New.CleanUp.ReferenceOTU99878 Coral.1.27F\_812586  
> GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTTAGATGAAACCAGAGGATGCTTGCATCCTCTTGTGATTCTAAACCTAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCGTATGCAGGGGGACAACAGTTGGAAACGACTGCTAATACCGCATAAGCGCACAGCTTCGCATGGAGCAGTGTGAAAAGATTTATCGGCATACGATGGACCCGCGTCTGATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGAGGATGAGGAGCCGGCCGGAGAGGGTGAACGGCCACATTGGGACTGAGAAACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGAACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCGGCAGGGAAGAAAGGCCTGAAAGGGAGACGACGGTACCTGACTAAGAAGCCCCGGCTAACTCCGTACCAGCCGCCGCGGTAATAC

Thanks  
Francesco

---

<div class="post-metadata">

### Author: ![Fra](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/f/a6a055/32.png) [@Fra](https://forum.qiime2.org/u/Fra)
#### Post date: [October 19, 2021, 5:35am UTC](https://forum.qiime2.org/t/importing-fna-and-workflow/21109/2 "2021-10-19T05:35:39Z")

</div>

I have also tried to follow this tutorial ([Clustering sequences into OTUs using q2-vsearch](https://forum.qiime2.org/t/clustering-sequences-into-otus-using-q2-vsearch/1348)) with no luck.

---

<div class="post-metadata">

### Author: ![Fra](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/f/a6a055/32.png) [@Fra](https://forum.qiime2.org/u/Fra)
#### Post date: [October 19, 2021, 5:36am UTC](https://forum.qiime2.org/t/importing-fna-and-workflow/21109/3 "2021-10-19T05:36:28Z")

</div>

maybe @gregcaporaso can give me a hint? Thanks!

---

<div class="post-metadata">

### Author: ![annguyen9724](https://forum.qiime2.org/user_avatar/forum.qiime2.org/annguyen9724/32/11621_2.png) [@annguyen9724](https://forum.qiime2.org/u/annguyen9724)
#### Post date: [October 19, 2021, 7:37am UTC](https://forum.qiime2.org/t/importing-fna-and-workflow/21109/4 "2021-10-19T07:37:36Z")

</div>

you can try this :-?  
[https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494/11](https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494/11)

---

<div class="post-metadata">

### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [November 19, 2021, 1:38pm UTC](https://forum.qiime2.org/t/importing-fna-and-workflow/21109/5 "2021-11-19T13:38:07Z")

</div>

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.