I am running QIIME 2 through the Galaxy interface through docker and have just completed many of the tutorials. I am interested in doing eDNA metabarcoding analysis and have fastsanger.gz files that I want to import. I saw in a prior post that users recommended using SampleData[PairedEndSequencesWithQuality] and format at Paired End Fastq Manifest Phred33V2. I did this and am getting an error that says: "Fatal error: Exit code 1 ()"
Does anyone know how to import these fastqsanger.gz files?
Hello @grantchristian153, can you post a screenshot of exactly what error you received? Thank you.
Well, that sure is not a helpful error message! One thing you might try is uploading your fastq.gz file(s) to galaxy as a data collection then importing that collection as your SampleData[PairedEndSequencesWithQuality]. Some more instructions on how to do that can be found here with someone having a potentially similar issue (Basically sometimes QIIME 2 and Galaxy disagree a bit on the specifics of what a given thing should look like).
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.
