Hi there!
I am new for qiime2. I am trying to import my Illumina data. The company sent me two types of files for each treatment: forward and reverse fastaq.gz and merged file, also fastaq.gz. According to the company, demultiplexing has already been done.
The "look" of forward file is:
From what I recall, your column title should be: “forward-absolute-filepath” and there should be another column for “reserse-absolute-filepath” in your manifest.
Also I can see your R1 and R2 files are in the same column, it is likely the R2 file is a reverse sequencing file and should be in the revervse column. Ben
edit: Your sampleid should likely be MS1, MS2 and so on --> the MS1_R1 and MS1_R2 likely reflects the forward (R1) and reverse (R2).
@jwdebelius and @ben,
I got the error. I had hidden letter in my manifest file, and prepared again according to @ben suggestions. I saw the green letter