Hi,
I have raw data (without barcode and primer) run on Illumina paired-end platform looks like this: - DMX12345_L1_SAMPLE1_1.fq.gz - DMX12345_L1_SAMPLE1_2.fq.gz - DMX12345_L1_SAMPLE2_1.fq.gz - DMX12345_L1_SAMPLE2_2.fq.gz
As far as I understand, this file already demultipexed. So, I want to start with importing data and I used this command:
I have looked at Importing data tutorial, but but still unclear about the manifest format, do I need to create it together with importing paired end demultiplexed data?
You could certainly do that, but we have another alternative import method that will likely be less work! Check out the fastq manifest section of the importing guide for more detail!
Cool, so it looks like you saw that already! All the manifest is is a file with info about what files belong to what samples - you can write this in a text editor, or a spreadsheet program. When you “import” that manifest, the importer reads the contents of the file and says “okay, SAMPLE 1 is made up of /path/to/DMX12345_L1_SAMPLE1_1.fq.gz for the forward reads and DMX12345_L1_SAMPLE1_2.fq.gz for the reverse reads. ”
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