Importing Data - Phred Score


I’m attempting to import my data that was sequenced using Illumina Miseq. I’m using the Paired End Demultiplexed option. The program reads my manifest just fine, but I get this error:

An unexpected error has occurred:

  Decoded Phred score is out of range [0, 62].

See above for debug info.

Does anyone know of a way to figure this out so that I may continue?


Hey there @chrmille - can you provide a little more info for us?

  • What version of QIIME 2 are you running?
  • What is the exact command or commands you are running? Copy and paste please!
  • Can you provide the first ~10 lines of one of your files you are importing?

The error indicates that there is an issue with at least on Phred quality score in these reads being out of the known acceptable range. Were these paired-end reads already joined?

Thanks! :t_rex: :qiime2:

Maybe you need to use Phred 33 instead of 64 for importing.

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