# importing data - phared score

**URL:** https://forum.qiime2.org/t/importing-data-phared-score/24482
**Category:** User Support
**Tags:** import
**Created:** [October 19, 2022, 4:53am UTC](https://forum.qiime2.org/t/importing-data-phared-score/24482 "2022-10-19T04:53:21Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![karina\_F](https://forum.qiime2.org/user_avatar/forum.qiime2.org/karina_f/32/14340_2.png) [@karina\_F](https://forum.qiime2.org/u/karina_F)
#### Post date: [October 19, 2022, 4:53am UTC](https://forum.qiime2.org/t/importing-data-phared-score/24482/1 "2022-10-19T04:53:21Z")

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Hi!

I’m attempting to import my data that was sequenced using Illumina Miseq. I’m using the Paired End Demultiplexed option. The program reads my manifest just fine, but I get this error:

```auto
An unexpected error has occurred:

  Decoded Phred score is out of range [0, 62].

See above for debug info.

```

Does anyone know of a way to figure this out so that I may continue?

Thanks!

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### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [October 19, 2022, 2:51pm UTC](https://forum.qiime2.org/t/importing-data-phared-score/24482/2 "2022-10-19T14:51:47Z")

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Hello Karina,

Welcome to the forums! :qiime2:

> [@karina\_F](#):
>
> ```auto
> Decoded Phred score is out of range [0, 62].
> 
> ```

Looks like this uses the PHRED 64 offset. I'm not sure this is supported by Casava 1.8 paired-end demultiplexed fastq, but it is supported by the [Fastq manifest format](https://docs.qiime2.org/2022.8/tutorials/importing/#fastq-manifest-formats). You can try using  
`--input-format PairedEndFastqManifestPhred64V2` and see if that resolves the issue with the q-score range.

Let us know if you have any questions.

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [November 19, 2022, 8:52pm UTC](https://forum.qiime2.org/t/importing-data-phared-score/24482/3 "2022-11-19T20:52:31Z")

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