# Importing data from ion-torrent run

**URL:** <https://forum.qiime2.org/t/importing-data-from-ion-torrent-run/7387>\
**Category:** User Support\
**Tags:** import\
**Created:** [December 11, 2018, 3:07pm UTC](https://forum.qiime2.org/t/importing-data-from-ion-torrent-run/7387 "2018-12-11T15:07:27Z")\
**Posts on this page:** 6\
**Page:** 1

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**Author:** ![Manoj](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/m/d9b06d/32.png) [@Manoj](https://forum.qiime2.org/u/Manoj)\
**Post date:** [December 11, 2018, 3:07pm UTC](https://forum.qiime2.org/t/importing-data-from-ion-torrent-run/7387/1 "2018-12-11T15:07:27Z")

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Hi,

I only started working on QIIME2 recently and have a question regarding importing data in to QIIME 2. I used to work on Qiime and I find it very difficult to do that in Qiime2.

I ran 96 samples with both 16s and ITS primers. I got a single fastq file from the sequencing provider and would like some advise to import the data.

Kindly advise.

Cheers,  
Manoj

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**Author:** ![Mdavrandi](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/m/6f9a4e/32.png) [@Mdavrandi](https://forum.qiime2.org/u/Mdavrandi)\
**Post date:** [December 11, 2018, 5:12pm UTC](https://forum.qiime2.org/t/importing-data-from-ion-torrent-run/7387/2 "2018-12-11T17:12:20Z")

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Hi Manoj,

I recently had a similar problem. My solution is below:

> [@Importing 454 data to run dada2 denoise-pyro](https://forum.qiime2.org/t/importing-454-data-to-run-dada2-denoise-pyro/7371/3):
>
> Thanks @Nicholas_Bokulich. Thats exactly where I got stuck, though I finally managed to work it out by doing the following: Demultiplexed data in QIIME 1; split\_libraries.py -m region1\_map.txt -f 454/1.TCA.454Reads.fna -q 454/1.TCA.454Reads.qual -b 10 -L 500 -l 380 -d --record\_qual\_scores -n 1000000 -o region1\_w\_Q\_split\_library Merged resulting .fna and .qual files by convert\_fastaqual\_fastq.py -f 454/region1\_w\_Q\_split\_library/seqs.fna -q 454/region1\_w\_Q\_split\_library/seqs\_filt…

Hope this helps

Best  
Mehmet

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**Author:** ![ChrisKeefe](https://forum.qiime2.org/user_avatar/forum.qiime2.org/chriskeefe/32/1907_2.png) [@ChrisKeefe](https://forum.qiime2.org/u/ChrisKeefe)\
**Post date:** [December 11, 2018, 5:13pm UTC](https://forum.qiime2.org/t/importing-data-from-ion-torrent-run/7387/3 "2018-12-11T17:13:33Z")

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**Author:** ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)\
**Post date:** [December 11, 2018, 8:39pm UTC](https://forum.qiime2.org/t/importing-data-from-ion-torrent-run/7387/4 "2018-12-11T20:39:31Z")

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> [@Manoj](#):
>
> I got a single fastq file from the sequencing provider and would like some advise to import the data.

depending on where the barcodes are, it sounds like you should follow [this tutorial](https://forum.qiime2.org/t/demultiplexing-and-trimming-adapters-from-reads-with-q2-cutadapt/2313/20).

Good luck!

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**Author:** ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)\
**Post date:** [December 11, 2018, 8:39pm UTC](https://forum.qiime2.org/t/importing-data-from-ion-torrent-run/7387/5 "2018-12-11T20:39:33Z")

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**Author:** ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)\
**Post date:** [January 12, 2019, 2:39am UTC](https://forum.qiime2.org/t/importing-data-from-ion-torrent-run/7387/6 "2019-01-12T02:39:34Z")

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