Hello, I am relatively new to qiime. I am using the version QIIME2 (2018.6) and I am facing problems with importing the data.
The data is paired-end sequencing data generated from HiSeq-PE 250. Each sample has two separate file one for forward and one for reverse in fq.gz format. The barcode reads have been removed.
I choose " PairedEndFastqManifestPhred64” to import data and I am running the examples supplied by the official website. But I face the warning message:
qiime tools import \
Error: no such option: --input-format
When i remove the option input-format, the warning message is:
qiime tools import
There was a problem importing pe-64-manifest:
Importing ‘SingleLanePerSamplePairedEndFastqDirFmt’ requires a directory, not pe-64-manifest
Could you help me, please?
Thank you in advance.