Importing data and input format

Hi @Fatemah,
Great question!

The Phred variations are explained well here.

The difference between manifest format and casava format is explained in our importing tutorial, or at least the practical difference for you: Casava format files have a specific naming pattern, otherwise both are for demultiplexed fastq data.

Phred64 is an older variation of the fastq quality scores, so unless if you know you are using old data then Phred33 is probably correct.

Phred33 and Phred64 have quality scores on different scales, so chances are you will get an error message if you try importing in the wrong format! So if you are importing without issue, you probably picked the correct format.

I hope that helps!