# Importing 454 raw reads
**URL:** https://forum.qiime2.org/t/importing-454-raw-reads/2469
**Category:** User Support
**Tags:** pending-development, 454, import
**Created:** [January 8, 2018, 4:05pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469 "2018-01-08T16:05:20Z")
**Posts on this page:** 8
**Page:** 1
### Author:  [@LauraMason](https://forum.qiime2.org/u/LauraMason)
#### Post date: [January 8, 2018, 4:05pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469/1 "2018-01-08T16:05:20Z")
Hello,
I would like to use Qiime 2 to analyze 454 data without using Qiime1 first to generate the .biom table. Can anyone think of a way to do this? Is there a way to convert the .fna file to .qza?
Thanks!
---
### Author:  [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [January 8, 2018, 8:39pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469/3 "2018-01-08T20:39:17Z")
Hi @LauraMason!
Do you have a way to get that data as a `fastq` file? While not yet supported, we are planning on updating our DADA2 plugin to handle 454 but it does need quality information.
If not, you might be able to use our `vsearch` pipeline for more typical clustering, but I don't think we have tested that particular use case for it yet. @Nicholas_Bokulich, do you see any reason that wouldn't work?
---
### Author:  [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [January 8, 2018, 8:44pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469/5 "2018-01-08T20:44:38Z")
The vsearch OTU clustering pipeline (see [this tutorial](https://forum.qiime2.org/t/clustering-sequences-into-otus-using-q2-vsearch/1348)) should work for 454 data.
The only issue I see is that there would be no 454-specific denoising steps. You could perform denoising steps with qiime1 first, then import the denoised fasta data into QIIME2 for clustering and downstream analysis.
---
### Author:  [@LauraMason](https://forum.qiime2.org/u/LauraMason)
#### Post date: [January 9, 2018, 7:03pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469/6 "2018-01-09T19:03:58Z")
Hello - the data is from a collaborator, so I would have to ask about the fastq. In the meantime, I will try that pipeline. Thanks!
---
### Author:  [@Joseph\_Sevigny](https://forum.qiime2.org/u/Joseph_Sevigny)
#### Post date: [January 18, 2018, 2:52pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469/7 "2018-01-18T14:52:42Z")
Hello, @LauraMason, @ebolyen
I have had good results with [GitHub - indraniel/sff2fastq: extract 454 Genome Sequencer reads from a SFF file and convert them into a FASTQ formatted output](https://github.com/indraniel/sff2fastq). It converts .sff files to fastq and retains the quality information. If you are need any help running it let me know. I also know a few scripts if it needs to be demultiplexed before being converted.
I have someone interested in running DADA2 with 454 data so I would like to see this supported!
Best,
Joe Sevigny
---
### Author:  [@LauraMason](https://forum.qiime2.org/u/LauraMason)
#### Post date: [January 19, 2018, 4:15pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469/8 "2018-01-19T16:15:06Z")
Hi Joe
I was able to perform demultiplexing and OTU picking in Qiime1 before moving my data into Qiime2 as a feature table. I may try that method next though to compare for myself OTU picking and the ASV method.
However, I have .fna files of reads, not .sff. Will this be an issue?
Cheers
Laura
---
### Author:  [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [January 19, 2018, 4:21pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469/9 "2018-01-19T16:21:18Z")
> [@LauraMason](#):
>
> I have .fna files of reads, not .sff. Will this be an issue?
Do you also have a separate file containing the quality scores? If yes, you will need to find a different converter ([qiime1 has one](http://qiime.org/scripts/convert_fastaqual_fastq.html)) to merge the .fna and qual scores into one fastq file.
---
### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [February 19, 2018, 10:21pm UTC](https://forum.qiime2.org/t/importing-454-raw-reads/2469/10 "2018-02-19T22:21:20Z")
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.