Hi! I am very new to Qiime2, and I have received 16S MiSeq data that I need to analyze. I received 3 files from the sequencing center - titled 5730-P1_S1_L001_I1_001, 5730-P1_S1_L001_R1_001 and 5730-P1_S1_L001_R2_001 so I assumed it is in Casava 1.8 format however I am still confused as to the files I got - I haven’t found topics on the forums showing how to correctly import using I1, R1 and R2.
I tried importing the R1 and R2 through Casava 1.8, viewed the quality plot, selected the trimming length and ran dada2, but I received an error message
An error was encountered while running DADA2 in R (return code -9), please inspect stdout and stderr to learn more.
I did it because I thought my data was already demultiplexed by the center but then I noticed that demux.qzv showed just 1 sample while there should be 45. Here is the quality plot. So this means that my data is multiplexed, right? Then how should I proceed?
Thank you and Merry Christmas!