# Import problem: Not a(n) QIIME1DemuxFormat file
**URL:** https://forum.qiime2.org/t/import-problem-not-a-n-qiime1demuxformat-file/21305
**Category:** Technical Support
**Tags:** import
**Created:** [November 11, 2021, 10:18am UTC](https://forum.qiime2.org/t/import-problem-not-a-n-qiime1demuxformat-file/21305 "2021-11-11T10:18:51Z")
**Posts on this page:** 3
**Page:** 1
### Author:  [@emiliomastriani](https://forum.qiime2.org/u/emiliomastriani)
#### Post date: [November 11, 2021, 10:18am UTC](https://forum.qiime2.org/t/import-problem-not-a-n-qiime1demuxformat-file/21305/1 "2021-11-11T10:18:52Z")
Hello buys,
I have troubles importing my fan file. This is what I have done:
1. I have my fastq file (single) already trimmed, suppose file1.fq
2. I trasforma it to fasta file with seqkit fq2fa file1.fq -o file2.fna
3. I tried to import it in qiime2 with the command: qiime tools import --input-path file2.fna --output-path seqs.qza --type SampleData[Sequences]
4. I obtained the following error:
There was a problem importing prova.fna:
file2.fna is not a(n) QIIME1DemuxFormat file
Here is test file I am using:
(qiime2-2021.8) emastriani@bilbo-01:~/MetaVic/Clustering$ more file2.fna
> SRR957824.2269 2269/1
> ATATCAGTGAAAATGCCCTGAAGGTAATGTACAGGCTCAATAAAGCGGGATACGAAGCCTGGCTGGTTGGCGGCGGCGTGCGCGACCTGTTACTCGGCAAAAAGCCGAAAG
> ATTTTGACGTAACCACTAACGCCACGCCTGAGCAGGTGC
> SRR957824.2269 2269/2
> ACTTCGATAATCTCCGGGCCAAACATCACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTT
> ACGTCAAAATCTTTCGGCTTTTTGCCGAGTAACAGGTCG
I am interested to import the fasta file because I need to practice with the procedure explained in [Clustering sequences into OTUs using q2-vsearch](https://forum.qiime2.org/t/clustering-sequences-into-otus-using-q2-vsearch/1348)
Thank you very much for your support.
Emilio
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### Author:  [@llenzi](https://forum.qiime2.org/u/llenzi)
#### Post date: [November 11, 2021, 3:08pm UTC](https://forum.qiime2.org/t/import-problem-not-a-n-qiime1demuxformat-file/21305/2 "2021-11-11T15:08:18Z")
Hi @emiliomastriani,
Did you download the sequences form sra?
This previous question may give you some help:
> [@question about importing SRA study as artifacts](https://forum.qiime2.org/t/question-about-importing-sra-study-as-artifacts/15800):
>
> Hi there, I am familiar with QIIME1 but relatively new with QIIME2. I have gotten my raw file in the past from a facility in the CASAVA pair ended demultiplexed format and I had no problem analyzing those files with both versions of qiime. I am now conducting an investigation of public data available in the SRA archive and I am having a time importing the raw data as QIIME2 artifacts. I have downloaded different projects (prefetch and fastq-dump commands in the SRA toolkit) and I don't think …
A general point, I would import the sequences fastq files, possibly as paired-end, if you have those (in your case you seems to have both R1 and R2 in the same file, so you need a script to split them into two files).
For the import, please have a look at the "Fastq manifest format" in the import guide:
[Importing data — QIIME 2 2021.8.0 documentation](https://docs.qiime2.org/2021.8/tutorials/importing/)
Then you would need to join your sequences as described at:
[https://docs.qiime2.org/2021.8/tutorials/read-joining/](https://docs.qiime2.org/2021.8/tutorials/read-joining/)
After that, you can use vsearch plug in to cluster your sequences as your initial point (starting form dereplicating the sample):
[https://docs.qiime2.org/2021.8/tutorials/read-joining/](https://docs.qiime2.org/2021.8/tutorials/read-joining/)
Hope it makes sense1
Cheers
Luca
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [December 12, 2021, 9:09pm UTC](https://forum.qiime2.org/t/import-problem-not-a-n-qiime1demuxformat-file/21305/3 "2021-12-12T21:09:04Z")
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