# Import Novaseq data

**URL:** https://forum.qiime2.org/t/import-novaseq-data/29467
**Category:** General Discussion
**Tags:** import
**Created:** [March 4, 2024, 9:59am UTC](https://forum.qiime2.org/t/import-novaseq-data/29467 "2024-03-04T09:59:54Z")
**Posts on this page:** 5
**Page:** 1

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### Author: ![gaudy\_93](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/g/54ee81/32.png) [@gaudy\_93](https://forum.qiime2.org/u/gaudy_93)
#### Post date: [March 4, 2024, 9:59am UTC](https://forum.qiime2.org/t/import-novaseq-data/29467/1 "2024-03-04T09:59:54Z")

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Good morning:  
I just received the novoseq sequences and am a bit lost. The files they have sent me are two files with the formats raw\_1.fastq.gz 【reads1 with barcode and primer】  
raw\_2.fastq.gz 【reads2 with barcode and primer】  
The format of the file opened it was showed in the image.  
What would be the best method to import this data?  
Best regards:  
Sergio

 ![image](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/5/3/535fdb4c96884c2018ffc91ec71bba8a136ceff3.png)

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### Author: ![gaudy\_93](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/g/54ee81/32.png) [@gaudy\_93](https://forum.qiime2.org/u/gaudy_93)
#### Post date: [March 4, 2024, 11:17am UTC](https://forum.qiime2.org/t/import-novaseq-data/29467/2 "2024-03-04T11:17:05Z")

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Sorry the reads are with barcode and primer removing

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### Author: ![colinvwood](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinvwood/32/17303_2.png) [@colinvwood](https://forum.qiime2.org/u/colinvwood)
#### Post date: [March 4, 2024, 5:24pm UTC](https://forum.qiime2.org/t/import-novaseq-data/29467/3 "2024-03-04T17:24:40Z")

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Hello @gaudy_93,

Did you have a chance to read through the [importing docs](https://docs.qiime2.org/2024.2/tutorials/importing/)?

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### Author: ![gaudy\_93](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/g/54ee81/32.png) [@gaudy\_93](https://forum.qiime2.org/u/gaudy_93)
#### Post date: [March 5, 2024, 2:40pm UTC](https://forum.qiime2.org/t/import-novaseq-data/29467/4 "2024-03-05T14:40:46Z")

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Yes, I read it but this format doesn't fit any of the ones you mention, that's why I was a bit stuck.

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### Author: ![buzic](https://forum.qiime2.org/user_avatar/forum.qiime2.org/buzic/32/16494_2.png) [@buzic](https://forum.qiime2.org/u/buzic)
#### Post date: [March 5, 2024, 3:27pm UTC](https://forum.qiime2.org/t/import-novaseq-data/29467/5 "2024-03-05T15:27:31Z")

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Hi @gaudy_93

If you look down the importing docs page that @colinvwood posted, there is a section called " Multiplexed paired-end FASTQ with barcodes in sequence" and I think that's the section you need.

After importing you can demultiplex with [cutadapt](https://docs.qiime2.org/2024.2/plugins/available/cutadapt/demux-paired/). Have a look around the forum, for example see [this post](https://forum.qiime2.org/t/importing-and-demultiplexing-sequence-data-quick-reference/14002) as a quick guide.

best,

Vic