NextSeq data isn’t demultiplexing well. Not sure how to proceed. Barcodes in our MiSeq are usually reverse complemented, but with the NextSeq runs they’re on the forward reads. 12 base pairs, but not the usual Golay with the EMP guidelines. Wondering if someone could help point me the way to see how I can get these imported correctly. Thank you very much. Ben
We formatted the data in such a way as we have a multiplexed file of forward and reverse fastq.gz files from the NextSeq. The different samples are within these files. This is the way we processed the EMP MiSeq files, but I think there’s some incompatibility with the way that NextSeq headers/files are being seen by Qiime2.
Praying to the mods, unqueue me.
Thanks mods, may you be blessed with the rains down in Africa